<p>The concentration of cloud condensation nuclei (CCN) is an essential parameter affecting aerosol-cloud interactions within warm clouds. Long-term CCN number concentration (NCCN) data are scarce, there are a lot more data on aerosol optical properties (AOPs). It is therefore valuable to derive parameterizations for estimating NCCN from AOP measurements. Such parameterizatios have been made earlier, in the present work a new one is presented. The relationships between AOPs, NCCN and particle number size distributions were investigated based on in-situ measurement data from six stations in very different environments around the world. The parameterization derived here depends on the scattering Ångström exponent (SAE), backscatter fraction (BSF) and total scattering coefficient (σ<sub>sp</sub>) of PM<sub>10</sub> particles. The analysis showed that the dependence of NCCN on supersaturation SS% is logarithmic:<br> N<sub>CCN</sub> ≈ ((287 ± 45)SAE<sub>10</sub>ln(SS%/(0.093 ± 0.006))(BSF − BSF<sub>min</sub>) + (5.2 ± 3.3))σ<sub>sp</sub>.<br> At the lowest supersaturations of each site (SS% ≈ 0.1) the average bias, defined as the ratio of the AOP-derived and measured NCCN varied from ~ 0.7 to ~ 1.5 at most sites except at a Himalayan site where bias was > 4. At SS% > 0.3 the average bias ranged from ~ 0.7 to ~ 1.3 at all sites. In other words, at SS% > 0.3 NCCN was estimated with an average uncertainty of approximately 30 % by using nephelometer data. The squared correlation coefficients between the AOP-derived and measured NCCN varied from ~ 0.5 to ~ 0.8. The coefficients of the parameterization derived for the different sites were linearly related to each other. To study the explanation of this, lognormal unimodal particle size distributions were generated and NCCN and AOPs were calculated. The simulation yielded similar relationships between the coefficients as in the field data. It also showed that the relationships of the coefficients are affected by the geometric mean diameter and width of the size distribution and the activation diameter.</p>