Atmospheric Chemistry and Physics Discussions www.atmos-chem-phys-discuss.net 1680-7367 1680-7375 9 2 2009 10.5194/acpd-9-6787-2009 http://www.atmos-chem-phys-discuss.net/9/6787/2009/ http://www.atmos-chem-phys-discuss.net/9/6787/2009/acpd-9-6787-2009.html http://www.atmos-chem-phys-discuss.net/9/6787/2009/acpd-9-6787-2009.pdf 6787 6825 2009-03-11 Comparison of analytical methods for HULIS measurements in atmospheric particles C. Baduel D. Voisin voisin@lgge.obs.ujf-grenoble.fr J. L. Jaffrezo Laboratoire de Glaciologie et Géophysique de l'Environnement (UMR 5183), Université Joseph Fourier-CNRS, Rue Molière, BP 96, 38 402 St Martin d'Hères, France Humic-Like Substances (HULIS) are a major contributor to the organic carbon in atmospheric aerosol. It would be necessary to standardise an analytical method that could be easily and routinely used for HULIS measurements. We present one of the first comparisons of two of the main methods in use to extract HULIS, using i) a weak anion exchanger (DEAE) and ii) the combination of two separation steps, one according to polarity (on C₁₈) and the second according to acidity (with a strong anion exchanger SAX). The quantification is performed with a DOC analyzer, complemented by an investigation of the chemical structure of the extracted fractions by UV-Visible spectroscopy. The analytical performances of each method are determined and compared for humic substances standards. These methods are further applied to determine the water extractable HULIS (HULIS_WS) and the 0.1M NaOH alkaline extractable HULIS (HULIS_T) in atmospheric aerosol collected in an Alpine Valley during winter time. This comparison shows that the simpler DEAE isolation procedure leads to higher recoveries and better reproducibility and should therefore be recommended. 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