We have developed a new laboratory nucleation setup to study binary homogeneous nucleation (BHN) of sulphuric acid and water (H<sub>2</sub>SO<sub>4</sub>/H<sub>2</sub>O). Here we provide a detailed evaluation of this new experimental setup and also discuss our preliminary results by comparing with other laboratory studies. H<sub>2</sub>SO<sub>4</sub> is produced from the SO<sub>2</sub>+OH →HSO<sub>3</sub> reaction and OH radicals are produced from water vapor UV absorption. The residual H<sub>2</sub>SO<sub>4</sub> concentrations ([H<sub>2</sub>SO<sub>4</sub>]) are measured at the end of the fast flow nucleation reactor with a chemical ionization mass spectrometer. The measured BHN rates (<i>J</i>) ranged from 0.02 and 550 cm<sup>−3</sup> s<sup>−1</sup> at the residual [H<sub>2</sub>SO<sub>4</sub>] from 10<sup>8</sup> to 10<sup>10</sup> cm<sup>−3</sup>, a temperature of 288 K and relative humidity (RH) from 6 to 23%; <i>J</i> increased with increasing [H<sub>2</sub>SO<sub>4</sub>] and RH. <i>J</i> also showed a power dependence on [H<sub>2</sub>SO<sub>4</sub>] with the exponential power of 3 to 8. These results are consistent with other laboratory studies under similar [H<sub>2</sub>SO<sub>4</sub>] and RH, but different from atmospheric field observations which showed that particle number concentrations are often linearly dependent on [H<sub>2</sub>SO<sub>4</sub>]. Both particle sizes and number concentrations increased with increasing [H<sub>2</sub>SO<sub>4</sub>], RH, and nucleation time, consistent with the predictions from nucleation theories. Particle growth rates were estimated between 28 to 127 nm h<sup>−1</sup>, much higher than those seen from atmospheric field observations, because of the higher [H<sub>2</sub>SO<sub>4</sub>] used in our study. While these experimental results demonstrate a validation of our laboratory setup, there are also technical difficulties associated with nucleation studies, including wall loss and H<sub>2</sub>SO<sub>4</sub> measurements.