Atmos. Chem. Phys. Discuss., 11, 16861-16894, 2011
www.atmos-chem-phys-discuss.net/11/16861/2011/
doi:10.5194/acpd-11-16861-2011
© Author(s) 2011. This work is distributed
under the Creative Commons Attribution 3.0 License.
Review Status
This discussion paper has been under review for the journal Atmospheric Chemistry and Physics (ACP). Please refer to the corresponding final paper in ACP.
Formation of hydroxyl radical from San Joaquin Valley particles extracted in a cell-free solution
H. Shen and C. Anastasio
Department of Land, Air and Water Resources, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA

Abstract. Previous studies have suggested that the adverse health effects from ambient particulate matter (PM) are linked to the formation of reactive oxygen species (ROS) by PM. While hydroxyl radical (OH) is the most reactive of the ROS species, there are few quantitative studies of OH generation from PM. Here we report on OH formation from PM collected at an urban (Fresno) and rural (Westside) site in the San Joaquin Valley (SJV) of California. We quantified OH in PM extracts using a cell-free, phosphate-buffered saline (PBS) solution with or without 50 μM ascorbate (Asc). The results show that generally the urban Fresno PM generates much more OH than the rural Westside PM. The presence of Asc at a physiologically relevant concentration in the extraction solution greatly enhances OH formation from all the samples. Fine PM (PM2.5) generally makes more OH than the corresponding coarse PM (PMcf, i.e., 2.5 to 10 μm) normalized by air volume collected, while the coarse PM typically generates more OH normalized by PM mass. OH production by SJV PM is reduced on average by (97 ± 6) % when the transition metal chelator desferoxamine (DSF) is added to the extraction solution, indicating a dominant role of transition metals. By measuring calibration curves of OH generation from copper and iron, and quantifying copper and iron concentrations in our particle extracts, we find that PBS-soluble copper is primarily responsible for OH production by the SJV PM, while iron often makes a significant contribution. Extrapolating our results to expected burdens of PM-derived OH in human lung lining fluid suggests that typical daily PM exposures in the San Joaquin Valley are unlikely to result in a high amount of pulmonary OH, although high PM events could produce much higher levels of OH, which might lead to cytotoxicity.

Citation: Shen, H. and Anastasio, C.: Formation of hydroxyl radical from San Joaquin Valley particles extracted in a cell-free solution, Atmos. Chem. Phys. Discuss., 11, 16861-16894, doi:10.5194/acpd-11-16861-2011, 2011.
 
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